Applications of Liquid Chromatography-Mass Spectrometry

Abstract

It is a sophisticated analytical technology with very high
sensitivity and specificity, the Liquid Chromatography-Mass
Spectrometry. Combining Liquid Chromatography (LC) with
spectroscopy is known as LC-MS (MS). It is possible to separate
components using the Liquid Chromatography (LC) technique,
which is followed by the transfer of the samples to Mass
Spectrometry (MS) where the detection, identification and
determination of masses may be done in the presence of other
elements. Quantitative and qualitative analysis of pharmaceutical
drug ingredients, intermediates, and related chemicals is
accomplished using LC-MS. in-vitro dissolution, bioequivalence,
bioavailability, and metabolite investigations are the
most common uses of LC-MS. It is also utilised in fundamental
research, agriculture, forensics and the food and beverage
sectors. LC-MS Instrumentation and uses of the LC-MS method
are briefly reviewed in this article.